Designing and constructing a micro flow injection system (μFIA) for phosphate determination in soft drinks

Lab. made micro flow Injection system (μFIA) was designed which supplied with two microcontroller UNO and Mega types. These two microcontroller (Arduino) were provided with home- made software programs.The first one, type UNO used to control home- made mini peristaltic pump to drive reagents to detector. The other one was Mega type which was used as a data-logger to manipulate and recording the results as peak height corresponding the concentration by using Microsoft Excel 2010 program. The diameter of the tubes was 0.2mm i.d which used in all parts of manifolds The proposed μFIA manifold was used for phosphate determination in soft drinks samples which supplied from local market. The best conditions for the determination of phosphate by Murphy method was studied. linearity, detection limit, r.s.d% were 5-30 μg/ml,0.09 μg/ml and 0.48% respectively. The phosphate concentrations in the different soft drink samples were in the rang 250-825 μg/ml by using the standard addition method in order to eliminate all expected interferences. The home -made Semi- automated micro-flow injection analysis (μFIA) system was applied successfully for phosphate estimation with simple, high sensitive and accurate.