Abstract
The paper uses a method that combines biological characteristics and 16S rDNA sequence characteristics to analyze potato scab bacteria collected from China. The study found that the obtained universal detection primers B1/B2 showed good specificity for all pathogenic strains, and could stably expand the target band, while non-pathogenic strains had no bands. The paper uses the spore dilution method to verify that the established detection method has a sensitivity of 20 pg·μL-1 for strain DNA, and the detection threshold for spores is about 4.0 CFU/μL. The genome amplification results of the test samples showed that the target bands were detected in the scab pathogen Streptomyces, diseased potato sample tissues, and diseased soil samples, but not the scab pathogen Streptomyces, healthy tuber tissues, non-sick soil samples and other strains None of the bands of interest were expanded, indicating that the method has good specificity. Therefore, we can conclude that the qualitative detection method of potato scab bacteria can realize rapid detection of strains, diseased tissues and soil samples.
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This article has been retracted by the authors following correspondence with IOP Publishing in which the authors claim the work is unreliable. The author's explanation follows:
"The material of the paper is bacteria, it is not be visible to the naked eyes, the material was contaminated by the drugs that out of date and got polluted, and it caused the serious mistake in the figure 2. The result of different strains of DNA by the primer in 3.3 was not match of our material. The figure 2 was the amplification results of different strains of DNA , and the fragment size of different strains were same in the result, but the result should not be same , so the result was wrong. When we continue to the study, we got new result about the figure 2. There was the amplification results of different strains of DNA by primer TXT in repeated experiment , and the result in the published article was wrong, the material was contaminated. We are afraid that other results were also incorrect in this paper."
IOP Publishing cannot verify this information as accurate, however in the interest of transparency and reproducibility, IOP Publishing agrees to retract this article. This notice will be updated if more information comes to light.