Abstract
Underutilized Oxyeleotris marmorata fish is abundant and widespread in Indonesia. The study aimed to use O. marmorata fish for peptone production using papain from dried latex of papaya fruit. The peptone was applied as nitrogen sources for bacterial growth. The resulted peptone was optimized at 50-65°C for 5-8 hr, using 0.1% of papain at pH 6.0. Characterization of peptone was based on the soluble protein content, N-amino content, % degree hydrolysis (DH), SDS PAGE profile and growth of bacteria Escherichia coli and Staphylococcus aureus. The results indicated that the optimum condition for hydrolysis was at 50°C for 7 hr (p < 0.05). Fish peptone soluble protein content was of 8.6 mg/mL, α-amino was 0.59%, and AN/TN 5.47%. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS PAGE) profile of peptone showed a major band with molecular weight between 17-28 kDa. Fish peptone effectiveness for E. coli and S. aureus growth was similar with commercial bacterial peptone.
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