Detection of Staphylococcus aureus in wound infection on the skin surface

Wound infection on the skin surface is colonized a wide variety of microorganisms. Microorganisms that cause inflammation of the skin surface is group of pyogenic bacteria. Staphylococcus aureus is one of the class of pyogenic bacteria that produce β-lactamase enzyme and eliminate the antibacterial activity, especially penicillin. The existence of this enzyme will destroy β-lactam ring, so that antibiotics become inactive. This study aimed to detect presence of Staphylococcus aureus on pus from wound infection on the skin surface. This study was descriptive qualitative. Three samples of pus was isolated and identified by culture and biochemical testing using RapID STAPH PLUS. The results of the study identified two isolates of Staphylococcus aureus with a probability >99.9% and 1 isolate of Staphylococcus hyicus with a probability 86.92%.