Effect of benzyl amino purin (BAP) and gibberellin acid (GA₃) to chlorophyll and antioxidant enzymes of soybean (Glycine max (L) Merill.) genotypes in response to inundation conditions

Indonesia's climate change issues cause the significant potential for inundation to occur when rainfall is too high in the area of soybean cultivation, which can give an impact on soybean productivity. The objective of this research was to measure the effect of growth regulators which can respond to soybean plant adaptation to inundation stresses. The influence of Benzyl Amino Purin (BAP) and Gibberellin Acid (GA₃), and their mixture on the chlorophyll and antioxidant defense system of three (Grobogan, Willis, and Detam-1) soybean genotypes has been conducted subjected to inundation condition in March 2018. The chlorophyll total by GA₃ was significantly increased at Detam-1 soybean genotype, while no change in other two soybean genotypes in GA₃, BAP, and the mixture compared to controls. Exogenous GA₃ enhanced the activity of superoxide dismutase (SOD) in Grobogan and Detam-1 soybean genotypes in response to inundation (72 h). On the other hand, peroxidase (POD) activity was enhanced on Grobogan with GA₃, and Detam-1 soybean genotypes with BAP applications when compared to control. Our result reveals that SOD together with POD activities was participate to protect Detam-1 soybean genotype from inundation condition.