Abstract
As an herbal plant, the many parts of black cumin are able to cope with various kinds of diseases including viruses. The need to provide bioactive ingredients in that time short is not accompanied by the availability of land and germplasm. With the existence of these problems, tissue culture techniques can be used to addressing the tissue. Callus culture is a heterotrophic cultivation. By paying attention to the large content of secondary metabolite compounds useful ones can be produced through callus induction techniques. Currently, the study has started using MS media, and modification of the type of tissue culture media by adding Picloram of 1 ppm combination with GA3 of 2 ppm can increased the percentage of callus growth. likewise affects the formation of phenolic and flavonoid content of black cumin callus.
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