Purification of Leucine aminopeptidase and its correlation with biochemical parameters in sera of subclinical hypothyroidism and hypothyroidism

The aim of this study purification of Leucine aminopeptidase (LAP) from hypothyroidism patients sera and its relation to thyroid hormones, lipid peroxidation levels of subclinical hypothyroidism and hypothyroidism patients, blood samples were collected from (50) healthy subject and (100) patients, consisted of fifty with hypothyroidism and fifty with subclinical hypothyroidism.. The purification is done by addition of ammonium sulfate, dialysis, anion- exchange chromatography and size-exclusion chromatography. FT3, FT4, TSH, LAP, peroxynitrite, malondialdehyde were determined. Results showed the precipitate and concentrated protein appeared five peaks in ion exchange column, LAP activity located in the first and second protein peak, also, results showed single peak for both first and second peaks after eluted in gel filtration chromatography following steps by using SDS-polyacrylamide gel electrophoresis. From current study, it is concluded hypothyroidism (LAP) have two isoenzymes, also, concluded a highly significant increase in LAP, MDA and peroxynitrite levels in the subclinical hypothyroidism and hypothyroidism patients when compared to the healthy subject, and their correlation with FT3, FT4, TSH in the patient group, when compared by the control group that indicate these parameters could be play an effect role in these diseases.