Identification of carbapenemases enterobacteriaceae producing gene blaVIM in clinical isolates

Carbapenemase enzymes play the most important role in the existence of CRE (Carbapenem-resistant Enterobacteriaceae). VIM is one of the carbapenemase enzymes encoded by the bla_VIM gene. The genes which are partially located in genetic mobile elements may facilitate the spread of the resistance to other bacteria. The aim of this study was to identify the bla_VIM gene in CRE isolated from infected patients in dr. Mohammad Hoesin Palembang. This descriptive observational laboratory study using 709 samples of Enterobacteriaceae isolated from various specimens of infected patients at dr. Mohammad Hoesin Palembang during the September-November period which would be identified as CRE using Vitek 2 Compact. 21 isolates which have been identified by CRE was taken by purposive sampling method to detect bla_VIM gene with conventional polymerase chain reaction (PCR). The PCR product was electrophoresed and visualized to see a single 390bp DNA band. Out of 709 isolates of Enterobacteriaceae, 88 (12.4%) isolates were identified as CRE. Only 21 isolates of CRE performed PCR. Three (14.3%) isolates carrying the blaVIM gene which found in Enterobacter sp. (N=2; 66.7%) and Klebsiella pneumoniae (n=1; 33.3%). In this study 14.3%, Enterobacteriaceae with CRE phenotype has bla_VIM gene genotype.