Cytotoxicity Assay of Ethyl Acetate Extract Shimeji (Lyophyllum shimeji (Kawam.) Hongo) and White Oyster Mushroom (Pleurotus ostreatus Jacq.) Against HCT-116 Cell Line

Cancer is a serious of health problem. Anticancer agent discovery is currently directed toward natural resources, including the edible mushrooms. The purpose of this research was to obtain the edible mushroom extract and to evaluate the bioactivity of the extracts in inhibiting the proliferation of colorectal cancer cell line (HCT-116) using MTT-based cytotoxicity assay. This experimental study was performed using 2 factorial Randomized Complete Design, where the first factor was the mushroom species i.e. Lyophyllum shimeji and Pleurotus ostreatus, while the second factor was concentration of the extract i.e. 0 (control), 1, 10, 100 and 1000 ppm. In this study, the proliferation inhibition percentage of HCT-116 cell line was measured and then analyzed by probit to determine the IC₅₀ value. The results showed mushrooms extract were cytotoxic for HCT-116 cell line, but only the concentration factor that influenced the proliferation inhibition of HCT-116 cell line. IC₅₀ values from shimeji and white oyster mushrooms extracts were 111.09 and 239.46 ppm, respectively. Thus, shimeji mushroom extract was more effective for proliferation inhibition HCT-116 cell line and potentially developed as natural anti-colorectal cancer in the future' drugs discovery.