Abstract
We previously developed micron-sized, femtoliter (fL) reaction chamber array made of polydimethylsiloxane and polyacrylamide. Downsizing of the reaction volume to fL level made it possible to carry out single-molecule assay (SMA) of biological reactions in a straightforward manner. In this review, preparation of the fL chamber array and its application to SMA of enzyme, DNA, and rotary motor protein under an optical microscope are described.