Quality of medical microscope Image at different lighting condition

A no- reference quality metrics is one of the challenging fields in image quality assessment. The aim of the research is measuring the quality of the microscope medical image such as blood smear and sample texture, at different lightness conditions by using two types of light sources are Tungsten and LED. To find the best light level at imaging, the no- reference quality metrics are calculated by using the histogram in a HL component in the wavelet transform. This measure is Compare with the other no-reference algorithms as entropy and average gradient by calculating the correlation coefficient between the subjective and objective methods. The results show that the proposed algorithm is a good measure of the quality of the medical microscope images at different lighting condition.