A new Cghd I gene with negative regulation on the synthesis of chaetoglobosin A

Chaetoglobosins have wide application prospect, but the low yields make it not widely used. The aim of this study was to obtain high-yielding strains and to find the genes regulating the synthesis of chaetoglobosins. A strain with yield increased to 115% was obtained through inserting a short-DNA randomly into the genome of Chaetomium globosum by agrobacterium. At the same time, inverse PCR was used to determine the insertion position of T-DNA. A new gene Cghd I with negative regulation on the synthesis of chaetoglobosin A was found, indicating that the detectable random insertability mutation technique established in this paper can be used to obtain high yield strains and find regulatory genes.