Abstract
Gold (Au) is one of the metals that is widely used for jewellery and electroplating. Although in the nanoparticle form, the waste that produced still has high economic value. Since its belong to heavy metals, gold nanoparticle waste need to be removed even in the very low concentration. One of the alternatives is utilized gold-resistant bacteria, namely Bacillus cereus S1. The purpose of our study was to determine the ability of B. cereus S1 to accumulates gold. Reconfirmation test of B. cereus S1 gold resistant ability was figured out using Minimal Salt Medium (MSM), 2% glucose and 0.1 ppm gold. The bioaccumulation process was used 1 ppm, 5 ppm, and 10 ppm concentration of gold; and the incubation periods were 6, 12, and 24 hours. Gold bioaccumulation performed by B. cereus S1 was measured using Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-EOS). The results showed that the longer incubation periods of B. cereus S1, the higher gold concentration will be accumulated at exposure concentration 1 ppm, 5 ppm and 10 ppm. B. cereus S1 showed good viability after 24 hours metal exposure.
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