Anti-leukaemia of fermented product of methanol extract Hyptis pectinata (L.) Poit leaf

Hyptis pectinata (L.) Poit is a traditional medicinal plant that has long been used in some countries such as in Northeast Brazil, Ghana, Thanzania and West Indies, while in Indonesia, not much is known. Hiptolida is the main compound of Hyptis pectinata (L.) Poit leaf which has potential as anticancer. Biotransformation by fermentation is one approach to increase bioactive activity of plants. This study aims to determine the anti-leukemia activity of fermented product of methanol extract of leaf Hyptis pectinata (L.) Poit. Fermentation is carried out by the addition of methanol extracts in the early of death phase of Apergillus niger culture on potato dextrose broth media and harvested in the mid-phase of death. The fermented product was purified to get crystals. The crystal obtained is 37.8 mg, white in the form of a needle with a melting point range of 102-103°C. Based on toxicity test on leukaemia cell P388, fermented product extract has IC₅₀ at: 84,21 μg/mL. While the toxicity test of methanol extract without fermentation obtained IC₅₀ at: 21,01 μg/mL. Based on UV spectroscopy analysis the fermented product has a wavelength absorption capacity of 249.8 nm which is much different from pure hyptolide. Based on the FTIR data, it was also found that the -OH group that widened in the band 3394 cm⁻¹ which previously was not present in the hyptolide compound, was thought to have formed a transformant compound. The results showed that the extract methanol leaves of Hyptis pectinata (L.) Poit has better anti-leukaemia activity compared to transformation from fermented products.