Abstract
Saccharum arundinaceum has strong drought tolerance, the abundant stress tolerance gene resource could be used in sugarcane breeding programs. Superoxide dismutase (SOD) is a crucial enzyme in reactive oxygen species (ROS) action, which are likely to function as components of a stress-signaling pathway. To investigate the gene of SOD, by using a homologous cloning strategy, a Cu/Zn-SOD cDNA sequence(NCBI accession number XM_ 002445626.1) in Sorghum was as probe to retrieve, aligned and spliced with Saccharum EST database, a full length of Cu/Zn-SOD gene named SaSOD-1b (NCBI accession number: KJ 002570) from Saccharum arundinaceum was cloned. The cloned gene sequence was 692bp, including 136bp 5' untranslated regions (UTR), 162bp ORF and 135bp 3' UTR, encoded 206 amino acids. By bioinformatics analysis, the cloned genic protein molecular weight was 20.78kDa, and pI was 5.32, the amino acid sequence owned the Cu/Zn-SOD conserved domain, and it had high similarity with other plants' Cu/Zn-SOD. The phylogenetic tree analysis indicated that the cloned gene was most closely relative with Sorghum Cu/Zn-SOD gene, this study could laid a foundation for further understanding the relation between SOD and Saccharum arundinaceum drought resistance.
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