The role of rhizobacteria to control rhizoctonia disease and to improvement plant growth of soybean on sub-optimal dry land

Rhizoctonia disease is caused by the Rhizoctonia solani fungus. that is a soil pathogen and is very dangerous because of its ability to persist in soil (soil borne pathogen) for a long time. Controlling by chemical not effective because they are have negative impact for example pollute the environment, harm non-target organism and harmful to humans. For that efforts are required alternative control an effective and environmentally friendly, one of them is by use bacteria PGPR. Some the genus of rhizobacteria often use are Pseudomonas fluorescens and Bacillus subtilis able to control a plant pathogen. The aims of research to understand the role of rhizobacteria potential in controlling rizoctonia disease and improvement plant growth of soybean. The result in vitro research showed that combination bacteria antagonistic P. fluorescens and B. subtillis provide better than single treatment, with inhibition is 88,1 % on 7 days after inoculations. While in vivo research showed that a combination of both the bacteria is also the best results with the disease insidence 12.4 % with frequency application 3 times, the control (not treatment) showed disease insidane 25,6 % with frequency application 4 times. The variables of compliance were N-total of tissues (%) shows that combination treatment bacteria better than the single treatment, with the N precentage is 0,499 % and heavy dry up to 4,12 %.