The Influence of PES (Provassoli's Enriched Seawater) media and modification of vitamin B₁₂ on technical culture for the growth of Sargassum sp.

One medium for algae culture is Provassoli's Enriched Seawater (PES), which is the best medium for most marine algae. In this study, enrichment media used with vitamin B12 elements were used to support the growth of Sargassum sp. This study was conducted to obtain a good concentration of vitamin B12 in Provassoli's Enriched Seawater (PES) media and its effect on the growth of Sargassum sp. This study uses an experimental method with Completely Randomized Design (CRD), There were 4 treatments for administering doses of vitamin B12 (2 g; 2.5 g; 3 g and controls) and 5 replications. If the treatment given gives a significantly different effect, it will be continued with Duncan's Multiple Distance Test (DMRT) to determine the differences between treatments. The results showed Provassoli 's Enriched Seawater (PES) media with a modification of vitamin B12 on technical culture media has a very significant effect (P <0.05) on the growth of Sargassum sp. Treatment with the addition of 3 grams of vitamin B12 is the best growth treatment. At the fourth week the daily growth rate is 0.33%, relative growth rate 0.027 gram and average value of chlorophyll a content of 2.295 mg/L.