Methods for determining the toxicity of technogenic materials used in road construction

A method for determining the toxicity of soil and technogenic materials used in road construction has been developed. It is proposed to determine the toxicity of technogenic substances used as building materials for the construction of road surfaces by using biotesting on Colpoda steinii infusoria based on the test reaction of the survival rate of these infusoria. Soil samples for toxicity determination were taken at different distances from the roadbed (5, 10, 20, 25, 50 m from the edge of the road surface). During the experiment, it was found that anyone of the tested soil samples taken at a distance of 25 and 50 m from the roadway is not toxic to soil infusoria, and even after three hours of water extraction in all experimental samples, the test objects survived. These data on toxicity confirm additional experiments conducted by another method of biotesting on test cultures. In this case, soft spring wheat (Triticum vulgare L.) variety Novosibirsk 29 and watercress (Lepidium sativum) variety Donskoy were used as test crops. Experiments with these test cultures showed complete identity of the results obtained for toxicity according to the proposed method using Colpoda steinii infusoria.