Turpentine oils as a basis for the creation of nanostructured lyosols: physicochemical characteristics and stability

Currently, one of the most common methods for the synthesis of dispersions based on turpentine in aqueous media is the preparation of its solutions in water using multi-stage chemical and/or physical treatment. In this case, the chemical composition is selected in such a way as to ensure the complete dissolution of hydrophobic substances in water. An example of solving such a problem is the use of an aqueous solution of the following composition: gum turpentine 43.0%; oleic acid 25.0%; castor oil 18.0%; caustic soda 3.0%. However, such methods do not allow setting and controlling dimensional parameters in the final product. In addition, in many cases, such a lyosol requires the addition of stabilizers and emulsifiers, which is determined by the purpose of the synthesized lyosol. Here we show the possibility of influencing the size parameter of the resulting particles in lyosol and their resistance to sedimentation in an aqueous solution. This result is achieved by homogenization using ultrasonic dispersion of a low-concentrated aqueous solution of turpentine, bubbling with various gases and subsequent filtration of the resulting emulsion through a membrane with a suitable pore size. This approach makes it possible to obtain at least 95% of nanoparticles from the total dry matter fraction. Compounds based on such lyosols, as a rule, have immunoreactogenic properties and can be used as an adjuvant component that increases the immunogenicity of inactivated vaccines, including for veterinary use.