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Evaluation parameters of in vitro morphogenesis in the process of replication of potato starting material

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Published under licence by IOP Publishing Ltd
, , Citation E Oves et al 2021 IOP Conf. Ser.: Earth Environ. Sci. 677 042030 DOI 10.1088/1755-1315/677/4/042030

1755-1315/677/4/042030

Abstract

In the process of replicating and growing the necessary volumes of in vitro material for original seed production, the period of formation of morphological structures and the achievement of microplants of standard characteristics is important. The existing method of regeneration assessment by the quantity of days from the moment of placement of micro stalks into a new nutrient medium does not allow a planned approach to the compilation and implementation of the clonal micropropagation program. The article proposes new methodological approaches to assessing in vitro potato material by the main phases of plant development in tissue culture: intensive growth (formation of 2-3 internodes), slow growth (4-6 internodes), and natural dying-off. The analysis of regenerative ability allows us to note that the effectiveness of the formation of morphological structures did not depend on the ripeness group of the studied variety samples. Presented results indicate the differentiated approach of varieties to the formation of morphological structures in the process of replication.

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10.1088/1755-1315/677/4/042030