Study of the influence of external factors on the inheritance of forms of dissociants Pseudomonas mandelii

In the course of studying the phase variations of the Pseudomonas mandellii strain IB-Ki14, the pH of the medium was found to be the main factor inducing the switching of dissociants on LB medium. Dissociants of the P. mandellii strain IB-Ki14 are stably inherited at pH 5.0-6.0. Dissociant cells differed in antioxidant and protease-producing abilities. In general, matte colony cells had higher antioxidant and proteolytic activity. At the same time, the cells of the mucous colonies had a higher extracellular Arg-X protease activity compared to the matte ones and, accordingly, were able to hydrolyze a larger amount of protein from the nutrient medium. An analysis of the Arg-X protease activity of the dissociant cell fractions revealed differences at the level of 0.35 M NaCl fraction (loosely bound to the cell residue), which is quite possibly associated with the localization of proteolysis in a specific subcellular region, which allows spatial control of the content of regulatory proteins.