Agronomic Characteristics, Yield Components and Polymorphic Markers between IRBL Kp-K60 and Ciherang for Developing Rice Resistant to Blast

Blast is one of the most devastating disesase in rice field in Indonesia which caused by Pyricularia oryzae. Utilising rice resistant variety is one of the most efective, economical dan eco-friendly solution for the disease. To develop rice resistant to blast is required resources of plant with resistance gene and popular variety as the parents. Introgressing gene pik in chromosome 11 from IRBL Kp-K60 into Ciherang was conducted using markers-assisted backcrossing. In this backcrossing program, selection process is a crucial part to gain the goal of the program. The goal of backcrossing program is to obtain plant which has all Ciherang characteristics with additional trait, resistance to blast. Some characteristics of the parents such as agronomic characteristics and yield components must be observed to assist phenotypic selection in the progeny. Moreover, applying DNA markers in backcrossing program will facillitate selection proccess to ensure that resistance gene is already transfered from donor into recepient. Thus, it is important to analyse polymorphic DNA markers between the parents. The aim of the study were to asses parental difference in agronomic characteristics and yields component and analyse polymorphic markers between Ciherang and IRBL Kp-K60 for developing rice resistant to blast disease. The result of study showed that several agronomic characteristics and yield component in Ciherang were more superior than IRBL Kp-K60.Flowering date of Ciherang was more longer than IRBL Kp-K60. Thirty six DNA markers were used in this study from various type of markers, namely CAPs, SSR and SNP. Some DNA markers were dominan and the others were codominan. There were fourthen DNA markers were polymorphics between the parents and can be used in the selection process in the progeny.