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Diversity of Arbuscular Mycorrhizal Fungi from Liberica Tungkal Jambi Coffee Plant Rhizosphere on Peatland

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Published under licence by IOP Publishing Ltd
, , Citation Elis Kartika et al 2019 IOP Conf. Ser.: Earth Environ. Sci. 391 012058 DOI 10.1088/1755-1315/391/1/012058

1755-1315/391/1/012058

Abstract

Arbuscular Mycorrhizal Fungi (AMF) diversity found in Liberica Tungkal Jambi coffee plant rhizosphere which planted on peatland in Tanjung Jabung Barat, Jambi Province has not been reported. The existence of various types of AMF is important to know because AMF can increase growth and production of coffee plant. This study aims to identify the diversity of AMF species from coffee plant rizosphere on peatlands in Tanjung Jabung Barat. The soil samples were taken from the coffee plant rhizosphere in Parit Pudin, Senyerang, and Serdang Jaya villages at a 0-20 cm depth. The isolation was carried out by Pacioni's wet screening technique and continued by centrifugation technique from Brunndret, while spore identification was performed based on morphological characterization and its response to Melzer's solution. The results showed that both in Parit Pudin and Senyerang villages found two genus, i.e. Acaulospora and Glomus, whereas in Serdang Jaya Village only Glomus was found. In Parit Pudin obtained 1 Acaulospora sp. and 6 Glomus sp., in Senyerang found 3 Acaulospora sp. and 6 Glomus sp., and in Serdang Jaya only found 6 Glomus sp. In Parit Pudin village, the spores abundance of Acaulospora sp. and Glomus sp. were 5 and 63 spores per 50 g of soil samples. In Senyerang village, the spores number of Acaulospora sp. and Glomus sp. were 57 and 49.5 spores per 50 g of soil samples, while in Serdang Jaya Village, the number spores of Glomus sp. as much as 46.83 spores per 50 g of soil samples. In all locations studied, there were various types of AMF with different numbers of spores. Spores found in soil samples showed variations in spore shape, spore color, attachment of hypha stalks, and spore walls.

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