Anti-inflammatory effect and chemical constituents of Microcos paniculata total flavone glycosides fraction

Microcos paniculata is an edible and medicinal plant in Lingnan, China. The present study investigated the anti-inflammatory effect and chemical constituents of its' total flavone glycosides (MpTFG) fraction. MpTFG (10, 15, 20 μg/mL) was applied to 1 μg/mL LPS-induced RAW 264.7 macrophages in vitro; and MpTFG (10, 20, 40 mg/kg body weight) was employed to xylene-induced ear edema test. As a result, 20 μg/mL MpTFG decreased the levels of pro-inflammatory cytokines IL-6, IL-1β and TNF-α in LPS-stimulated RAW 264.7 cells supernatant significantly, and 40 mg/kg MpTFG could effectively ameliorate xylene-induced mice ear swelling. The results showed that MpTFG had potential anti-inflammatory effect. Meanwhile, ten compounds were purified by making use of macro-porous resin, sephadex LH-20 gel column chromatography and semi-preparation HPLC successively. They were characterized to be vicenin-2 (i), isoschaftoside (ii), schaftoside (iii), vitexin (iv), vicenin-1 (v), isovitexin (vi), isoviolanthin (vii), nicotiflorin (viii), astragalin (ix), narcissoside (x) by ¹H-NMR, ¹³C-NMR and HRMS spectral analysis.