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Isolation and preservation of microfungi from Indonesia islands

Published under licence by IOP Publishing Ltd
, , Citation N F Wulandari 2019 IOP Conf. Ser.: Earth Environ. Sci. 308 012079 DOI 10.1088/1755-1315/308/1/012079

1755-1315/308/1/012079

Abstract

The research was aimed at utilizing more adequate methods of long-term preservation, cryopreservation (freezing at -80 °C). Samples obtained from three islands were used as sources of samples, i.e., Enggano, Riau, and Sumba. Direct isolation and single spore isolation were conducted to isolate the microfungi. All the cultures were grown on Potato Dextrose Agar (PDA). The growth rate of selected microfungi was calculated every day for a week. The growth rate for selected ten microfungi of each island, approximately 0.1-0.7 cm/day. All the cultures are maintained in InaCC (Indonesian Culture Collections), Cibinong, LIPI.

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10.1088/1755-1315/308/1/012079