Antioxidant activity of biocellulose-based films incorporated with powder of soursop leaves (Annona muricata L)

Biocelluloses are natural materials that are produced from Acetobacter sp through fermentation in coconut water as the medium. In this study, biocellulose-based edible film was used as the carrier film for the antioxidant agents (the soursop leave powder (Annona muricata L)). This leave is considered as a good source of natural antioxidant, while all parts of the fruit are used in traditional medicine. This work aimed to evaluate the antioxidant activity of biocellulose-based edible film added with soursop leave powder. The biocellulose was formed in slurry and leaves of fruit was crushed into powder. The edible films were prepared by mixing biocellulose slurry, carboxy methyl cellulose (CMC) as the homogenizer, and the leaves powder, casting, and drying in the oven at 40 °C for 24 h. The IC50 of methanol extract of leaves powder and edible film containing 0.2 g leaves powder were 89.89 mg/mL and 363.26 mg/mL, respectively. All methanol extracts of edible films incorporated with soursop leaves powder inhibited DPPH about 71.10-86.80% and they remained above 70% after storage in a plastic bag at room temperature for one month. The edible films also had the thickness of 0.040-0.067 mm, the solubility of 80.30-90.20% and all compounds of edible films were still available based on SEM and FTIR results.