Abstract
The partial pressure of hydrogen is a significant factor for dark fermentation. The effect of reduced hydrogen partial pressure as well as different operation condition was investigated in this study. The results showed that hydrogen production enhanced when partial pressure reduced. The reduction of hydrogen partial pressure (reduced by 20%, interval=2h) increased the efficiency of hydrogen production by 54%(202.15mL) compared with the control group. The kinetic parameters of hydrogen production show that the maximum hydrogen production (Pmax) and the maximum hydrogen production rate (Rmax) were increased by the reduction of hydrogen partial pressure. And the hydrogen production delay time (λ) decreased. Moreover, with the decrease of hydrogen partial pressure, ethanol content gradually increased, acetic acid/ethanol ratio decreased, total VFAs increased. The compositions of soluble microbial products as well as ecological factors were affected by hydrogen partial pressure.
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