The impact of dietary hydrolyzed collagen on bone's calcium deficiency of Rattus norvegicus

Type 1 Collagen is a protein consisting of polypeptides that constitutes organic structure of bones. During aging, the collagen content will decrease and lead to reduced bone strength as the result of the increased activity of bone resorption by osteoclasts cells. In the current experiment, the rat bones were conditioned into calcium deficiency state with low dietary calcium feeding. The hydrolyzed collagen and regular-sized of calcium phosphate (CaP) were later added into the feeding with certain composition as the intake to restore bone strength from the deficiency state. The quantitation of mineral groups and mineral compositions such as phosphorus (P), calcium (Ca), and magnesium (Mg) in bone matrix structure was conducted using Fourier Transform Infrared (FTIR) and Atomic Absorption Spectrophotometry (AAS). The results showed that the deposited mineral of deficiency state bones of rats fed with hydrolyzed collagen and CaP intake was higher compared tothose with CaP intake only, thus indicating that the dietary hydrolyzed collagen intake is essential method to restore the bone strength.