Application of in ovo injection of L-Glutamine for improving productivity of Indonesian native chicken: hatchability and hatching time

A study was aimed to improve productivity of the Indonesian Native Chicken (INC) by the technique of in ovo injection (IOI) of L-Glutamine (Gln), which hatchability and hatching time were measured. A 300 fertile egg (45.26±3.65 g) of INC were used and arranged as Randomized Block Design within 3 different incubators as blocks, and 5 treatment groups of 20 eggs: negative control (P0), positive control (P1-0.5 ml sterilized saline), 2.50 (P2), 5.0 (P3) and 7.5 (P4) mg Gln in 0.5 ml sterilized saline. The injections were conducted into the albumen at d 7 of incubation. IOI of Gln resulted in a significantly heavier weight of the newly hatched chickens (NHC), Hatchability of the P1 (60.83 ± 7.52%) and P2 (62.50 ± 2.89 %) was significantly higher (P<0.01) compared to those of the other groups; On the other hand, the embryo mortality in P1 and P2 were significantly lower compared with the other three groups, which mostly occurred before d 17 of incubation. It is concluded that IOI of 2.5 mg Gln of INC increased hatchability, decreased the duration of incubation time and the heavier weight of NHC would apparently have a better performance compared to that of the untreated eggs.