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Terpenoid as Antibacterial Produced by Endophyte Fusarium oxysporum LBKURCC41 from Dahlia variabilis Tuber

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Published under licence by IOP Publishing Ltd
, , Citation F Piska et al 2020 J. Phys.: Conf. Ser. 1655 012034 DOI 10.1088/1742-6596/1655/1/012034

1742-6596/1655/1/012034

Abstract

Fusarium oxysporum LBKURCC41 is an endophytic isolated from Dahlia variabilis tubers from Padang Luar, West Sumatra. The strain was fermented using modified Huangs' media, ie using corn as a carbon source for 15 days. The purpose of this study is to isolate secondary metabolites from LBKURCC41 strains. The filtrate from the liquid fermentation media was extracted using EtOAc:MeOH at a ratio of 95:5 to 50:50. Purification using fast chromatography was performed with a mixture of BAW (5:1:4). The structure was elucidated by, FTIR and 1H NMR spectroscopy. The isolate was identified as a sesquiterpenoid, with weak antimicrobial activity against E.coli and S. aureus with 2.1 mm clear zone and concentration of 10 ppm.

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10.1088/1742-6596/1655/1/012034