Influence of Multiple Short Highly Energetic X-ray Pulses on the Biosynthetic and Physiological Activity of the Strain Trichoderma reesei M7

In order to increase the biosynthetic capacity of the Trichoderma reesei-M7 strain – a notorious cellulase enzyme producer – the experimental biological object to be studied was exposed to the impact of the highly energetic X-ray pulses, produced by the dense plasma focus device (DPF). The X-ray pulses thus produced can have exceptionally high density of the flow of X-photons. The pulsed mode of irradiation fundamentally differs from the continuous when considering its effect on biological objects – they can rapidly absorb great amounts of radiation in a glimpse, leading to a qualitatively different interaction with their matter. In the experiments done the survivability was determined, as well as the vegetative biomass, four types of cellulase activity and the quantity of total protein after the treatment of the spores suspension (2.105 CFU/ml) of Trichoderma reesei M7 with this pulsed X-radiation. The absorbed doses were in the range of 7 to 45000 mSv. It was noticed that the survivability of the strain, if compared with the control samples, drops, reaching 57% of the initial value (of the untreated spores) for the sample with 45010 mSv of absorbed dose. From the data presented below it can be concluded that three of the samples with high absorbed doses, namely with 11754, 19439 and 42917 mSv, demonstrated increases their cellulase activities. In the case of 42917 mSv the increment of the endoglucanase activity, compared to the control, reaches 41,1%, FPA rises with 30,3%, and the β-glucosidase activity nearly doubles – and increase of 92,7%.