Preliminary Study of Scandium-46 Labeled Composite (Hydroxyapatite-Chitosan-Collagen) Biodistribution in Rats Bone Implant Model

Research related to bone fractures is currently focused on accelerating healing time with fewer complications. In some cases related to biological and mechanical factors that interfere with the healing process, it will take a longer time to heal. Hydroxyapatite (HA) is a promising material used as a scaffold for bone implants with various advantages. The in vivo biodistribution of Sc-46 labeled composite (HA-Chitosan-Collagen) remains unclear. In this research, Scandium-46 was prepared as a non-carrier free radioisotope solution by irradiating 100 mg Sc2O3 target in TRIGA 2000 Reactor Bandung. In vivo experiment was performed on Sprague Dawley rats weighing approximately 250 g. Rats bone implant model was divided into two groups with n = 3 per time point. The Sc-46 labeled composite (HA-Chitosan-Collagen) have implanted to rats femur 10 mg with radioactivity 10 μCi. Rats were euthanized using accepted protocol and all organs were counted for radioactivity using Wipe Test Counter with NaI(Tl) detector. The percent of radioactivity measured per gram of tissue weight (%ID/g). Biodistribution results showed that Sc-46 labeled composite (HA-Chitosan-Collagen) using the bone-implant method were significantly different compared with the normal bone for 1, 3, and 8 days of the time interval with p<0.05. These observations suggest that Composite (HA-Chitosan-Collagen) is available for bone implants and remains at the implant site until bone recovery.