The efect of lactobacillus reuteri probiotic to improve the amount of il 23 and il 22 cytokine on mus muscullus of postpartum model inducted by staphylococcus aureus

The childbed infection was an inflammation of all the genitalia organ on childbed period caused by aerobic and anaerobic bacteria. One of them was Stapylococcus aureus which could attack and survive to live in epithelial and endothelial cells, and recognized by Antigen Precenting Cell (APC). It would release cytokines as a trigger for T naive activation to produce Th1, Th2, Treg and Th 17. Interleukin-23 (IL 23) issued by APC would make T naïve differentiated into Th17 which released IL17 and IL 22 as a natural medicine being an antibiotic alternative studied. One of them was probiotic (Lactobacillus reuteri). This research aimed to prove the effect of L. reuteri probiotic to improve the amount of IL-23 and IL-22 cytokines to Mus muscullus of childbed model inducted by S. aureus. The research method was True Experimental through post test Only Control group in vivo the tenth day 40 Balb/c pregnant mice divided into 8 groups K-HI, K-HIII, K + HI, K + HIII, P1HI, P1HIII, P2HI and P2HIII (the first and third day of negative control group, the positive/given S. aureus on day 1 and day 3, the first and third day of L. reuteri group, the first and third day of L. reuteri + S. aureus group). Then, performed the surgery to obtain cardiac blood. Furthermore, it would produce blood plasma after doing centrifugation followed by measurement of IL 23 and IL 22 amount using ELISA method kit with catalog number M2300 and M2200 of R & D brand. The data were analyzed using Independent Sample T test through SPSS software for Windows 23. The comparison result between the negative control (K-) and the treatment group on the first day proved that the amount of IL 23 and IL 22 cytokines were increased. The comparison between the observed groups on the first day proved that the amount of IL 23 and IL 22 cytokines were increased. The result of comparative experiment between P1 and P2 on the first day the amount of IL 23 cytokine was increased, but the amount of IL 22 cytokine was not increased. It was not statistically significant. Meanwhile, the comparison results on the third day proved that the amount of IL 23 and IL 22 cytokines were increased, but in P1 group the amount of IL 22 cytokine was decreased. The giving of L. reuteri probiotic could increase the amount of IL 23 and IL 22 cytokines on childbed mus muscullus induced by S. aureus. Therefore, probiotics could be useful during postpartum period to improve the body immunity so that the infection during childbed period could be avoided.