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The application of barcode DNA rbcL gene for identification of medicinal plants: red jabon and gofasa

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Published under licence by IOP Publishing Ltd
, , Citation Sundari et al 2019 J. Phys.: Conf. Ser. 1146 012030 DOI 10.1088/1742-6596/1146/1/012030

1742-6596/1146/1/012030

Abstract

Red jabon and gofasa plants has potential as a medicinal plant. One of the potential drugs of these two plants as anti-cholesterol purpose of this study was to identify the red jabon and gofasa plants in Ternate Island using rbcL gene of DNA barcode. The method in this study consists of total DNA isolation using ZymoBiomic (Zymo Research DNA Extraction) Kit, DNA amplification using rbcL gene, sequencing and BLASTn analysis. Based on barcode DNA rbcL, the red jabon plants has similarities 99% Cephalanthus occidentalis, Mitragyna speciosa and Ocherreuclear sp. for gofasa plants has 99% resemblance with Vitex glabrata, Vitex megapotamica, Vitex trifolia and Vitex negundo at NCBI database. The application of rbcL barcode DNA is effective to identify plants at the family level up to the genus.

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