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Immobilization of Candida rugosa lipase by adsorption-crosslinking onto corn husk

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Published under licence by IOP Publishing Ltd
, , Citation A Nuraliyah et al 2018 IOP Conf. Ser.: Mater. Sci. Eng. 345 012042 DOI 10.1088/1757-899X/345/1/012042

1757-899X/345/1/012042

Abstract

Corn husk is one of the agricutural waste that has not been used optimally. corn husk waste allows to be used as immobilized support for biocatalyst because it is easy to obtain, available abundant, renewable and easy to decompose. This research was conducted in two phases, namely the adsorption of enzyme immobilization on the support, followed by cross- linking between the enzyme and support through the addition of glutaraldehyde. The optimum conditions for cross-linked adsorption immobilization using support of corn husk were achieved at concentrations of 0,75 mg / ml at 4 hour reaction time. The biggest unit activity value is obtained at 2,37 U / g support through 0.5% glutaraldehyde addition.

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10.1088/1757-899X/345/1/012042