Isolation and toxicity test of Bacillus thuringiensis from Sekayu region soil, South Sumatra on Spodopteralitura

This study aimed to obtain bacterial isolates B. thuringiensis potential as a biological control against pests Spodoptera litura. The research was conducted at the Laboratory of Pest and Disease Department, Agricultural Faculty of Sriwijaya University, Campus Inderalaya Ogan Ilir, South Sumatera, from March to June 2017. The study was conducted with survey method and laboratory trial. The results showed that of the 50 soil samples from three villages selected through morphological observation, reaction staining, KOH test, catalase test, producing 13 bacterial isolates. Screening of the 13th toxicity of the isolates suspected B. thuringiensis against S. litura larvae was investigated. Based on the toxicity screening test the following facts were obtained: five isolates ie KJ₂M₂, KJ₃E1, KJ₃JB₁, KJ₃D₃ and KJ₃D₅ were lower toxicity than Dipel, two isolates ie KJ₃K₄ and KJ₃D₃ which had the same toxicity to Dipel. Five isolates the KJ₃E₃, KJ₃BW₅, KJ₃JB₅, KJ₃D₁ and LC₂, LC₃ known to have effectiveness until the seventh day reached 40%. There was one isolate that is KJ₃BW₅ which was more effective compared to Dipel as comparison.