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The following article is Open access

DHM (Digital Holography Microscope) for imaging cells

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Published under licence by IOP Publishing Ltd
, , Citation Yves Emery et al 2007 J. Phys.: Conf. Ser. 61 1317 DOI 10.1088/1742-6596/61/1/260

1742-6596/61/1/1317

Abstract

Light interaction with a sample modifies both intensity and phase of the illuminating wave. Any available supports for image recording are onlysensitive to intensity, but Denis Gabor [P. Marquet, B. Rappaz, P. Magistretti, et. al. Digital Holography for quantitative phase-contrast imaging, Optics Letters, 30, 5, pp 291-93 (2005)] invented in 1948 a way to encode the phase as an intensity variation: the "hologram". Digital Holographic Microscopy (DHM) [D. Gabor, A new microscopic principle, Nature, 1948] implements digitally this powerful hologram. Characterization of various pollen grains and of morphology changes of neurones associated with hypotonic shock demonstrates the potential of DHM for imaging cells.

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10.1088/1742-6596/61/1/260