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The following article is Open access

Multisegment one-step RT-PCR fluorescent labeling of influenza A virus genome for use in diagnostic microarray applications

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Published under licence by IOP Publishing Ltd
, , Citation A V Vasin et al 2011 J. Phys.: Conf. Ser. 291 012006 DOI 10.1088/1742-6596/291/1/012006

1742-6596/291/1/012006

Abstract

Microarray technology is one of the most challenging methods of influenza A virus subtyping, which is based on the antigenic properties of viral surface glycoproteins – hemagglutinin and neuraminidase. On the example of biochip for detection of influenza A/H5N1 virus we showed the possibility of using multisegment RTPCR method for amplification of fluorescently labeled cDNA of all possible influenza A virus subtypes with a single pair of primers in influenza diagnostic microarrays.

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10.1088/1742-6596/291/1/012006