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Journal of Instrumentation Volume 6 January 2011 Create an alert RSS this journal

D Georgieva et al 2011 JINST 6 C01033 doi:10.1088/1748-0221/6/01/C01033

Evaluation of Medipix2 detector for recording electron diffraction data in low dose conditions

OPEN ACCESS 12th International Workshop on Radiation Imaging Detectors (IWORID)

D Georgievaa, J Jansenb, I Sikharulidzea, L Jianga, H W Zandbergenb and J P Abrahamsa

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Abstract References

Part of 12th International Workshop on Radiation Imaging Detectors (IWORID)

The drive for elucidation of important macromolecular structures to high resolution in their 3D native or near-native state places continuously higher demands on the quality of the experimental data. For instance, recording of diffraction patterns good enough for structural studies from cryo-preserved bio-macromolecules at low dose conditions remains challenging and highly desirable. The emergence of hybrid pixel detectors opens up new possibilities for direct electron detection and superior detector performance. Here, we report on the characteristics of the Medipix2 detector in diffraction studies, with a special focus on the reliability of the intensities acquired in very low dose conditions. Diffraction data recorded on a Medipix2 detector were assessed in refinement analysis. R-factors lower than 10% were obtained from data recorded at electron dose of 0.05 el/Å2. The reproducibility of the data was also shown to be high, given the correlation coefficient of the intensities being higher than 0.9970. The contrast that could be achieved at very low dose conditions was at least an order of magnitude better than that of image plates, based on a direct comparison.

Keywords

Radiation damage evaluation methods

Pixilated detectors and associated VLSI electronics

Neutron diffraction detectors

Photon detectors for UV, visible and IR photons (vacuum)

PACS

87.64.Bx Electron, neutron and x-ray diffraction and scattering

36.20.Hb Configuration (bonds, dimensions)

87.15.B- Structure of biomolecules

87.64.Ee Electron microscopy

Subjects

Soft matter, liquids and polymers

Atomic and molecular physics

Medical physics

Biological physics

Dates

Issue 01 (January 2011)

Received 30 October 2010, accepted for publication 11 November 2010

Published 11 January 2011



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