Abstract
Optically monitoring biosensing procedures based on the dynamics of an aqueous solution containing functionalized superparamagnetic beads in the application of the external rotating magnetic field has been developed for a rapid, highly sensitive, and inexpensive bioassay. Typically, the dynamics of micrometer diameter beads is observed by conventional optical microscopes. For greater affinity to biomolecules, there is a demand which necessitates the use of nanometer sized superparamagnetic beads, comparable size to actual biomolecules. However, a limited amount of work for monitoring the dynamics of nanometer sized beads has been performed thus far due to the maximum resolution of microscopes. Here, we propose a novel protocol enabling us monitor the dynamics of nanometer-diameter beads via change in the optical transmittance.
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