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Structure and dynamics of hemeproteins using X-ray Absorption Spectroscopy

Alessandro Arcovito1, Paola D'Angelo2 and Stefano Della Longa3

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Experimental investigations on protein dynamics are usually carried out in solution; however, in most cases, the determination of protein structure is done by crystallography that relies on the diffraction properties of the protein arranged in a three-dimensional lattice. X-ray Absorption Spectroscopy (XAS) is an ideal tool to investigate the deep relationships between structure and dynamics, on a wide class of metal containing proteins, including hemeproteins. In fact, this technique can be used either as a structural refinement of X-ray diffraction on the same crystal, or as an independent tool to study structural dynamic properties both in crystal and solution. Different examples will be presented and discussed, with particular emphasis onto the original contribution due to the application of the MXAN software package to the analysis of XANES spectra.


PACS

87.14.E- Proteins

87.15.H- Dynamics of biomolecules

87.15.N- Properties of solutions of macromolecules

87.15.B- Structure of biomolecules

87.80.-y Biophysical techniques (research methods)

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Dates

Issue 1 (2009)



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    Alessandro Arcovito et al 2009 J. Phys.: Conf. Ser. 190 012195

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