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DNA–protein interactions and bacterial chromosome architecture

REVIEW ARTICLE

Joel Stavans1 and Amos Oppenheim2

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REVIEW ARTICLE

Bacteria, like eukaryotic organisms, must compact the DNA molecule comprising their genome and form a functional chromosome. Yet, bacteria do it differently. A number of factors contribute to genome compaction and organization in bacteria, including entropic effects, supercoiling and DNA–protein interactions. A gamut of new experimental techniques have allowed new advances in the investigation of these factors, and spurred much interest in the dynamic response of the chromosome to environmental cues, segregation, and architecture, during both exponential and stationary phases. We review these recent developments with emphasis on the multifaceted roles that DNA–protein interactions play.


PACS

87.15.K- Molecular interactions; membrane-protein interactions

87.14.E- Proteins

87.14.G- Nucleic acids

87.16.Ka Filaments, microtubules, their networks, and supramolecular assemblies

87.16.Sr Chromosomes, histones

87.15.B- Structure of biomolecules

Subjects

Biological physics

Dates

Issue 4 (December 2006)

Received 20 September 2006, accepted for publication 30 November 2006

Published 22 December 2006

 
Rosette picture of supercoiled domain architecture of the nucleoid.


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