Greg Lakatos et al 2005 Phys. Biol. 2 166 doi:10.1088/1478-3975/2/3/004
First passage times of driven DNA hairpin unzipping
Greg Lakatos1, Tom Chou2, Birger Bergersen1 and Gren N Patey3
Show affiliationsWe model the dynamics of voltage-driven transport of DNA hairpins through transmembrane channels. A two-dimensional stochastic model of the DNA translocation process is fit to the measurements of Mathé, who pulled self-hybridized DNA hairpins through lipid-embedded α-hemolysin channels. As the channel was too narrow to accommodate hybridized DNA, dehybridization of the hairpin became the rate-limiting step of the transport process. We show that the mean first passage time versus voltage curve for the escape of the DNA from the transmembrane channel can be divided into two regions: (1) a low-voltage region where the DNA slides out of the pore in reverse and without undergoing significant dehybridization, and (2) a region where the DNA dehybridizes under the influence of the applied voltage and translocates across the membrane.
- PACS
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87.16.Uv Active transport processes
87.85.Qr Nanotechnologies-design
- Subjects
- Dates
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Issue 3 (September 2005)
Received 15 June 2005, accepted for publication 22 August 2005
Published 12 September 2005
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First passage times of driven DNA hairpin unzipping
Greg Lakatos et al 2005 Phys. Biol. 2 166
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