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Optically integrated microfluidic systems for cellular characterization and manipulation

Robert W Applegate Jr1, Dawn N Schafer1, Wafa Amir1, Jeff Squier1, Tor Vestad2, John Oakey2 and David W M Marr3

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Expanding interest in microfluidic techniques for biomedical applications has driven the recent need for micro-integrated optics capable of both traditional characterization and emerging optical manipulation techniques. We discuss here how ultrafast laser micromachining can be used to create optical waveguides directly within microfluidic systems. We then utilize this fabrication approach to create a unique microfluidic platform for optical characterization and sorting of cells and particles. This new platform employs optically fabricated waveguides to scatter and refract light from individual particles, allowing accurate in situ size detection and sorting within a microfluidic channel.


PACS

87.80.Fe Micromanipulation of biological structures

42.82.Et Waveguides, couplers, and arrays

87.85.Va Micromachining

47.85.Np Fluidics

85.85.+j Micro- and nano-electromechanical systems (MEMS/NEMS) and devices

42.82.Cr Fabrication techniques; lithography, pattern transfer

Subjects

Fluid dynamics

Electronics and devices

Instrumentation and measurement

Optics, quantum optics and lasers

Medical physics

Biological physics

Nanoscale science and low-D systems

Dates

Issue 8 (August 2007)

Received 15 January 2007, accepted for publication 2 March 2007

Published 23 July 2007



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