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Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability

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Published 16 November 2006 Published under licence by IOP Publishing Ltd
, , Citation Mariano Bossi et al 2006 New J. Phys. 8 275 DOI 10.1088/1367-2630/8/11/275

1367-2630/8/11/275

Abstract

We demonstrate the breaking of the diffraction resolution barrier in far-field fluorescence microscopy by photoswitching ensembles of optically bistable organic molecular markers from a non-fluorescent to a fluorescent state and back. The photoswitching is accomplished by an isomerization reaction of a photochromic compound serving as a reversible energy acceptor of a fluorescent compound. The surpassing of the diffraction barrier with power levels of only a few hundred W cm−2 of continuous wave irradiation is evidenced both in the effective point spread function and in the fluorescence images of test samples.

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10.1088/1367-2630/8/11/275