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Single-Molecule Assay of Biological Reaction in Femtoliter Chamber Array

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Published 20 August 2009 Copyright (c) 2009 The Japan Society of Applied Physics
, , Citation Ryota Iino et al 2009 Jpn. J. Appl. Phys. 48 08JA04 DOI 10.1143/JJAP.48.08JA04

1347-4065/48/8S2/08JA04

Abstract

We previously developed micron-sized, femtoliter (fL) reaction chamber array made of polydimethylsiloxane and polyacrylamide. Downsizing of the reaction volume to fL level made it possible to carry out single-molecule assay (SMA) of biological reactions in a straightforward manner. In this review, preparation of the fL chamber array and its application to SMA of enzyme, DNA, and rotary motor protein under an optical microscope are described.

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