Polymethylhydrosiloxane (PMHS) has been investigated as a candidate material for microfluidic chips. The ability to modify the surface of PMHS by hydrosilation is particularly advantageous for separation processes. The chemical modification of PMHS is verified by diffuse reflectance infrared Fourier transform (DRIFT) analysis, and the modified PMHS is shown to be stable when exposed to extreme pH conditions between 2 and 9. Spectrophotometer measurements show that PMHS exhibits over 40% transmittance for ultraviolet (UV) wavelength as low as 220 nm, indicating viability for sensor applications based on UV absorption. The UV transmittance is furthermore observed to be insensitive to thickness for specimens tested between 1.6 mm and 6.4 mm thick. Full curing of PMHS liquid resin occurs between 48 and 72 h at 110 °C with no secondary additives. Casting of microscale features is achieved by using soft lithography methods similar to established techniques for fabrication based on polydimethylsiloxane (PDMS). Microchannels approximately 100 µm wide and 50 µm deep are also demonstrated by carbon dioxide laser ablation, with uniform channels produced using an energy dose of 0.2 mJ mm⁻¹ with respect to line length. Other basic functional requirements for microfluidic chips are discussed, including the ability to bond PMHS substrates by plasma treatment.