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An optical Moiré technique for cell traction force mapping

Xiaoyu Zheng and Xin Zhang1

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Cells alter their shape and morphology and interact with their surrounding environment. Mechanical forces developed by cells to their surrounding environments are fundamental to many physiological processes, such as cell growth, division, migration and apoptosis. In this paper, a novel optical Moiré based biomechanol force sensor was developed for cell traction force mapping. We utilized coherent laser beams to illuminate periodic polymeric substrates where isolated cells were cultured. We demonstrated one-dimensional and two-dimensional traction force mapping via optical Moiré for both cardiac myocytes and vascular smooth muscle cells. The magnification effect of the Moiré fringe pattern permits a real time monitoring of the mechanical interaction between isolated cells and their underlying periodic polymeric structures.


PACS

87.80.-y Biophysical techniques (research methods)

87.19.Ff Muscles

87.19.R- Mechanical and electrical properties of tissues and organs

87.19.Hh Cardiac dynamics

Subjects

Instrumentation and measurement

Medical physics

Biological physics

Dates

Issue 12 (December 2008)

Received 28 May 2008, in final form 2 September 2008

Published 30 October 2008



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