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3D sheath flow using hydrodynamic position control of the sample flow

Hironobu Sato, Yutaka Sasamoto, Daisuke Yagyu, Tetsushi Sekiguchi and Shuichi Shoji

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3D sheath flow was realized using hydrodynamic position control of the sample flow. The symmetric microgrooves formed on the channel walls were utilized to generate local directional streams. The sample introduced into the grooved area was shifted to the center region of the microchannel, and 3D sheath flow was formed passively. Using CFD (computational fluid dynamics) simulation, the flow shift area was designed to achieve 3D sheath flow no longer than 500 µm in channel length. Sample flow shift behavior was observed by using a confocal microscope. Since the structure of the inlets was very simple, it was possible to fabricate an in-plane multi-sample 3D sheath flow device. As a demonstration, a two-sample 3D sheath flow device was fabricated. The separated two-sample 3D sheath flow configuration was clearly observed.


PACS

47.60.-i Flow phenomena in quasi-one-dimensional systems

47.11.-j Computational methods in fluid dynamics

47.85.Np Fluidics

47.85.Dh Hydrodynamics, hydraulics, hydrostatics

Subjects

Fluid dynamics

Computational physics

Dates

Issue 11 (November 2007)

Received 21 May 2007, in final form 3 August 2007

Published 3 October 2007



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