Chia-Yen Lee et al 2005 J. Micromech. Microeng. 15 1215 doi:10.1088/0960-1317/15/6/011
Chia-Yen Lee1, Gwo-Bin Lee2,3, Jr-Lung Lin2, Fu-Chun Huang2 and Chia-Sheng Liao3
Show affiliationsThe present paper reports a fully automated microfluidic system for the DNA amplification process by integrating an electroosmotic pump, an active micromixer and an on-chip temperature control system. In this DNA amplification process, the cell lysis is initially performed in a micro cell lysis reactor. Extracted DNA samples, primers and reagents are then driven electroosmotically into a mixing region where they are mixed by the active micromixer. The homogeneous mixture is then thermally cycled in a micro-PCR (polymerase chain reaction) chamber to perform DNA amplification. Experimental results show that the proposed device can successfully automate the sample pretreatment operation for DNA amplification, thereby delivering significant time and effort savings. The new microfluidic system, which facilitates cell lysis, sample driving/mixing and DNA amplification, could provide a significant contribution to ongoing efforts to miniaturize bio-analysis systems by utilizing a simple fabrication process and cheap materials.
Issue 6 (June 2005)
Received 30 December 2004, in final form 1 March 2005
Published 29 April 2005
Chia-Yen Lee et al 2005 J. Micromech. Microeng. 15 1215
J Dutton and D B Rees 1967 Br. J. Appl. Phys. 18 309
L O Vilarinho and C Fanara 2004 Meas. Sci. Technol. 15 67
Arbab I Arbab 2003 Class. Quantum Grav. 20 93
Andy Adler 2004 Physiol. Meas. 25 227
Winfried Michaelis et al 2004 Metrologia 41 L29
Jorge Llacer et al 2003 Phys. Med. Biol. 48 183
D L Livesey and C L Smith 1953 Proc. Phys. Soc. A 66 689
J B Seth et al 1928 Proc. Phys. Soc. 41 29
Emanuele Fiorani et al 2003 J. Phys. A: Math. Gen. 36 L101